
© Daniel Razansky, University and ETH ZurichA new imaging method can capture images of vasculature deep in the brains of mice. A conventional widefield fluorescence image of the mouse brain taken non-invasively in the visible light spectrum is shown on the left, while the non-invasive localization-based DOLI approach operating in the NIR-II spectral window is shown on the right.
WASHINGTON —
Researchers have developed a new technique that allows microscopic fluorescence imaging at four times the depth limit imposed by light diffusion. Fluorescence microscopy is often used to image molecular and cellular details of the brain in animal models of various diseases but, until now, has been limited to small volumes and highly invasive procedures due to intense light scattering by the skin and skull.
"Visualization of biological dynamics in an unperturbed environment, deep in a living organism, is essential for understanding the complex biology of living organisms and progression of diseases," said research team leader Daniel Razansky from the
University of Zurich and
ETH Zurich, both in Switzerland. "Our study represents the first time that 3D fluorescence microscopy has been performed fully noninvasively at capillary level resolution in an adult mouse brain, effectively covering a field of view of about 1 centimeter."
In
Optica, The Optical Society's (
OSA) journal for high impact research, the researchers describe their new technique, which is called diffuse optical localization imaging (DOLI). It takes advantage of what is known as the second near-infrared (NIR-II) spectral window from 1000 to 1700 nanometers, which exhibits less scattering.
"Enabling high-resolution optical observations in deep living tissues represents a long-standing goal in the biomedical imaging field," said Razansky. "DOLI's superb resolution for deep-tissue optical observations can provide functional insights into the brain, making it a promising platform for studying neural activity, microcirculation, neurovascular coupling and neurodegeneration."
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