Many civilians were reportedly killed in an explosion and building collapse in west Mosul.
A Pentagon spokesman on March 30 said the U.S. military will soon release a video showing IS fighters herding people into a building, then firing from the structure to bait coaltion forces.
The comments come as the U.S. military responds to criticism from within Iraq and internationally over a separate incident in which as many as 240 civilians are believed to have been killed.
"What you see now is not the use of civilians as human shields," said Colonel Joe Scrocca, a spokesman for the coalition. "Now it's something much more sinister."
He said militants are "smuggling civilians so we won't see them" into buildings and then attempting to draw an attack.
He said he was working on declassifying a video showing militants conducting such an operation.
Human rights group Amnesty International, Pope Francis, and others have urged for better protection for civilians caught in the war, with calls intensifying after a separate March 17 explosion in the Mosul al-Jadida district, killing scores of people.
The U.S. military previously acknowledged that coalition planes probably had a role in the explosion and subsequent building collapse, but it said the ammunition used was insufficient to explain the amount of destruction observed.
Officials said they suspect the building may have been booby-trapped or that the damage may have been caused by the detonation of a truck bomb.
U.S.-backed forces are attempting to push IS fighters out of west Mosul after having liberated the less-populated eastern part of Iraq's second-largest city.
Scrocca estimated that some 1,000 militants remain in west Mosul, their last stronghold in Iraq, down from 2,000 when the assault was launched on February 19.
They are facing about 100,000 Iraqi government forces, he added.
Comment: The Pentagon is damned if they do, damned if they don't. What they say above is plausible - that's the way ISIS operates. The Jihadis in Aleppo, and all over Syria, do the same or similar things. But the U.S. military will have to do a LOT to clean up their image if they want to really fight terrorism, and do so in a way that keeps what little honor they have intact. Decades of callous disregard for civilian life can't just be washed away. Funny thing is, ISIS knows that, which is why these tactics are so effective.