Cordero James Brady, 29, gave his children ice cream laced with the laxatives then shared the video with his 5.6million followers on his monetised channel 'CJ SO COOL'.
The prank appeared to make the children physically sick, but Brady jokes as one of them says 'my stomach hurts' by responding 'oh my goodness, this boy's stomach is broken'.
© YouTube/CJ So CoolHis partner also plays pranks on the children that are then uploaded
Despite them clearly being in some discomfort and trying to close the bathroom door he continues filming, ignoring their pleas.
A police spokesman told MailOnline: 'There was a case that was investigated in 2016 involving laxatives and this individual.
'It was a joint investigation with Clark County Child Protective Services. It was submitted to the DAs office and it was recommended as a criminal case.'
The video was only removed last week when it was highlighted to YouTube by babe.net.
The YouTube Channel has been reinstated but the video is no longer on there. Brady continues to prank his children.
He has lit fireworks while they sleep, carried out a drowning baby prank on his girlfriend and she has pranked him by pretending one of their children had a pencil stuck in their eye.
Dr Kortney Peagram from anti-child abuse charity Bulldog Solution, said: 'Harming your child on purpose with laxatives is child abuse. That's child endangerment.'
She added: '[he is] 'harming his children and bullying them to pull pranks on each other, it is really disturbing.
'He's modelling inappropriate behavior and encouraging it by laughter... He's teaching his children that it's okay to harm each other for a good laugh.'
YouTube said: 'Content that endangers minors is unacceptable to us and we have strict policies prohibiting child endangerment and harmful and dangerous content. We remove content that violates our policies as soon as we're made aware of it.'
But not much is ever mentioned about the exploitative, narcissistic, pedophilic world of children's pageants. If this article outlines child abuse, then children forced into pageants is certainly child abuse, yet it's hardly mentioned what those children go through!!