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Thursday's designations include nine new officials and an updated entry on Freddy Bernal, previously placed on the sanctions list as the former mayor of Caracas. Bernal is currently Venezuela's minister of urban agriculture.
US sanctions are also targeting three members of the National Electoral Council: Socorro Hernandez, Sandra Oblitas and Carlos Quintero.
Second Vice President of the Constituent Assembly Elvis Hidrobo, Culture Minister Ernesto Villegas, and Venezuela's ambassador to Italy Julian Rodriguez were also on the list announced Thursday.
Washington has refused to recognize Venezuela's elections for the Constituent Assembly, a body convened to amend the country's constitution. The US has accused Venezuelan President Nicholas Maduro of "dictatorship" and called for the end of his "illegitimate rule."
Comment: Washington wouldn't 'recognize' democracy if it smacked them in the face. As the leader of the new Constituent Assembly Delcy Rodriguez has said about these 'concerns',
Al Jazeera: Your government's decision to create a legislative superbody that you preside over is being described by many all over the world as a consolidation of a dictatorship. Yet you say that it's being done to bring about peace and dialogue in Venezuela. How is that possible?
Delcy Rodriguez: Well, you're referring to a dictatorship, correct? Since Commander Chavez came to power in 1998, Venezuela has been going through a process of consolidation of a participatory democracy. And this participatory democracy has been reinforced with the National Constituent Assembly elections.
There is nothing more democratic than consulting the people, nothing more democratic than listening to the people, nothing more democratic than the people deciding over their development, their destiny
Since the Constituent Assembly was established on July 30th, and we have to point it out, it wasn't created by pointing fingers, but through the universal, direct and secret vote of more than eight million Venezuelans.
Treasury Secretary Steven Mnuchin called the sanctioned officials "complicit in Maduro's attempts to undermine democracy," Reuters reported.
The Constituent Assembly was inaugurated in August 2017, following months of demonstrations organized by the opposition. Over 100 people were killed in the course of the protests.
Under the sanctions, the blacklisted officials are barred from traveling to the US, any assets they may have in the US will be frozen, and Americans are prohibited in doing business with them.
While targeting government officials and members of the Constituent Assembly, Washington has stopped short of banning the use of dollars in buying and selling Venezuelan oil, or embargoing oil imports into the US, Venezuela's biggest customer.
We tell you what you can do, when you can do it, where you can do it, how you can do it, and with whom you can do it. It's called freedom.
If the US is trying to make the whole world hate them (and definitely not for their "freedom"), they're doing a great job.