© Julien de Rosa / ReutersFrench President Emmanuel Macron and his wife Brigitte Macron
The petition, started two weeks ago on Change.org by painter and author Thierry Paul Valette, is demanding that no public funds be set aside for the position of First Lady for Mrs Macron.
While on the campaign trail, Macron announced his intention to create the position for his wife in order to clarify her status but promised no public funds would be used to pay her.
The centrist candidate romped home to victory with his newly formed En Marche party in May, becoming the youngest president in French history.
"Brigitte Macron currently has a team of two or three aides, as well as two secretaries and two security agents.That's enough," the petition reads.
Parliamentary opponents of Macron have previously protested against the plan during a time of government cutbacks. Parliament is also about to pass a new law designed to prevent MPs from hiring family members as assistants.
The petition wants to make clear that it is not an attack on Mrs Macron or her abilities.
"We fiercely denounce all sexist attacks against Brigitte Macron and we do not question her skills."
But that, "we can not decently endorse the initiative of a specific statute to the wife of President Macron."
Comment: UPDATE: The French president's wife, Brigitte Macron, will not be given an official "first lady" title or her own budget, the French government has said following a petition against a proposed change to her status.
The Élysée has made no official announcement, but officials were forced to react after the petition opposed to the president's spouse having an official title, status and budget was signed by more than 275,000 people in two weeks.