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Playing with fire: Engineered immune cells elicit broad response to HIV in mice using CRISPR

Immune Cells
Unlike so many other deadly viruses, HIV still lacks a vaccine. The virus — which continues to infect millions around the world — has proven especially tricky to prevent with conventional antibodies, in part because it evolves so rapidly in the body. Any solution would require coaxing the body into producing a special type of antibody that can act broadly to defeat multiple strains of the virus at once.

This week, scientists at Scripps Research moved closer to attaining that holy grail of HIV research with a new vaccine approach that would rely on genetically engineered immune cells from the patient's body.

In experiments involving mice, the approach successfully induced broadly neutralizing antibodies — also called bnabs — that can prevent HIV infection, says principal investigator James Voss, PhD, of Scripps Research. The study appears in Nature Communications.

Voss and his team showed in 2019 that it was possible to reprogram the antibody genes of the immune system's B cells using CRISPR so the cells would produce the same broadly neutralizing HIV antibodies that have been found in rare HIV patients.

Comment: In using CRISPR towards the described HIV vaccine it would seem that these scientists are quite simply playing with fire. But what else is new?

See also:


Brain

An old rat with no brain has raised some very interesting questions

lab rat
© Stock photo
The researchers had no idea how strange their lab rat was until, in a routine procedure, they scanned its head

Yes, R222 was only a rat. A rat that turned out to have no brain. But here's the thing: R222 had lived a normal life as a lab rat for two whole years. According to rat specialists, that's like 70 human years.

Researchers were, to say the least, puzzled.

The story begins with a scientist scanning the brains of "very old" lab rats as part of a study on aging. Except that subject R222, otherwise a conventional rat, didn't seem to have a brain. The brain cavity had collapsed and filled with fluid (hydrocephalus).

We can see from the photo that where the control rat has brain, R222 has fluid:

Comment: There are baffling human examples of brain anomalies too:


Info

Scientists find unexplained light in space

New Horizons spacecraft
© NASA / Johns Hopkins University Applied Physics Laboratory/Southwest Research Institute
Scientists have used the New Horizons spacecraft, billions of miles from Earth, to measure the darkness of space.
Look up at the night sky and, if you're away from city lights, you'll see stars. The space between those bright points of light is, of course, filled with inky blackness.

Some astronomers have wondered about that all that dark space--about how dark it really is."

Is space truly black?" says Tod Lauer, an astronomer with the National Optical Astronomy Observatory in Arizona. He says if you could look at the night sky without stars, galaxies, and everything else known to give off visible light, "does the universe itself put out a glow?"

It's a tough question that astronomers have tried to answer for decades. Now, Lauer and other researchers with NASA's New Horizons space mission say they've finally been able to do it, using a spacecraft that's travelling far beyond the dwarf planet Pluto. The group has posted their work online, and it will soon appear in the Astrophysical Journal.

New Horizons was originally designed to explore Pluto, but after whizzing past the dwarf planet in 2015, the intrepid spacecraft just kept going. It's now more than four billion miles from home — nearly 50 times farther away from the Sun than the Earth is.

That's important because it means the spacecraft is far from major sources of light contamination that make it impossible to detect any tiny light signal from the universe itself. Around Earth and the inner solar system, for example, space is filled with dust particles that get lit up by the Sun, creating a diffuse glow over the entire sky. But that dust isn't a problem out where New Horizons is. Plus, out there, the sunlight is much weaker.

To try to detect the faint glow of the universe, researchers went through images taken by the spacecraft's simple telescope and camera and looked for ones that were incredibly boring.

Cassiopaea

Surprising similarities between the human brain and the Universe

cerebellum
© University of Bologna
Left: section of cerebellum, with magnification factor 40x, obtained with electron microscopy (Dr. E. Zunarelli, University Hospital of Modena); right: section of a cosmological simulation, with an extension of 300 million light-years on each side (Vazza et al. 2019 A&A).
An astrophysicist at the University of Bologna and a neurosurgeon at the University of Verona compared the network of neuronal cells in the human brain with the cosmic network of galaxies... and surprising similarities emerged

In their paper published in Frontiers in Physics, Franco Vazza (astrophysicist at the University of Bologna) and Alberto Feletti (neurosurgeon at the University of Verona) investigated the similarities between two of the most challenging and complex systems in nature: the cosmic network of galaxies and the network of neuronal cells in the human brain.

Comment: See also: And check out SOTT radio's: MindMatters: Interview with Ken Pedersen: Quarks, DNA, Consciousness - It's All Information, Always Has Been

As well as:


Brain

Researchers use MRI technology to show telepathy between people

MRI Tech
Transferring thoughts, feelings, emotions, or one person invoking some sort of biological response in another, one that can be measured using modern day medical equipment like Magnetic Resonance Imaging (MRI), was the subject of a 2006 study published by several researchers in The Journal of Alternative and Complementary Medicine, titled Evidence for Correlations Between Distant Intentionality and Brain Function in Recipients: A Functional Magnetic Resonance Imaging Analysis.

The study found that distant intentionality (DI), which is defined as sending thoughts at a distance (telepathy) from a 'sender' is actually correlated with an activation of certain brain regions of the 'receiver.'

The study used eleven "healers" who do work in this area, and 11 other people who did not claim to be healers, but had some sort of special connection with the healer.

Attention

Planet of the Apes? - Scientists used human genes to make monkey brains bigger

Monkey Brain
© HEIDE ET AL./ MPI-CBG/GETTY
In an experiment that could portend a real-life Planet of the Apes situation, scientists spliced human genes into the fetus of a monkey to substantially increase the size of the primate's brain. And it worked.

Researchers from Germany's Max Planck Institute of Molecular Cell Biology and Genetics in Germany and Japan's Central Institute for Experimental Animals introduced a specifically human gene, ARHGAP11B, into the fetus of a common marmoset monkey, causing the enlargement of its brain's neocortex. The scientists reported their findings in Science.

The neocortex is the newest part of the brain to evolve. It's in the name — "neo" meaning new, and "cortex" meaning, well, the bark of a tree. This outer shell makes up more than 75 percent of the human brain and is responsible for many of the perks and quirks that make us uniquely human, including reasoning and complex language.

Bizarro Earth

Researchers find the possible remnants of a long-debated "missing" tectonic plate

Jonny and Spencer
© University of Houston
Jonny Wu (left) and Spencer Fuston used mantle tomography to investigate whether a controversial lost tectonic plate called Resurrection once made up part of the Pacific Ocean floor.
The ground beneath our feet might seem solid, but Earth's tectonic plates are always shifting. Sometimes, they even disappear. In a study published last month in the Geological Society of America Bulletin, researchers announced new evidence in favor of one such missing plate, dubbed the Resurrection plate after Alaska's Resurrection Peninsula. The Resurrection plate has long been a subject of controversy among geologists, who debate whether the Pacific Ocean once sported an extra plate that slid beneath what is now northern Canada 40 to 60 million years ago.

Using a technology called mantle tomography — akin to a CT (computerized tomography) scan of Earth's mantle — a team of geologists identified a rock slab under the crust of northern Canada that could be a remnant of the Resurrection plate. They then used 3D imaging technology to unfold and raise this "Yukon slab" back to the surface while winding back the geological clock. They found the Resurrection plate neatly plugged a gap between two known plates, the Kula and the Farallon, and also aligned with volcanic belts along the coasts of Alaska and Washington that typically form at plate boundaries.

The tomography technique used to resurrect the Resurrection plate could be a useful tool for finding other disappeared plate boundaries, according to the authors. These rediscovered boundaries could, in turn, help identify new volcanoes and mineral deposits and even refine models of past climate.

Info

Follow-up on recent NEO objects

Below you can find a selection of some objects for which we recently made follow-up observations at the "Osservatorio Salvatore di Giacomo, Agerola, ITALY" (MPC code L07; Observers E. Guido, A. Catapano, F. Coccia) while they still were on the NEOCP list. More details about the telescope, the magnitude, number of images & exposition, asteroid speed & PA etc. are on the images. Click on each image for a bigger version. All the processing has been made with TYCHO software by D. Parrott.

2020 VX5 (neocp designation C3WZUQ2) is an Apollo-type asteroid discovered by G96 Mt. Lemmon Survey on November 15, 2020. This asteroid has an estimated size of 55 m - 120 m (H=23.4) and it had a close approach with Earth at about 29 LD (Lunar Distances = ~384,000 kilometers) or 0.074 AU (1 AU = ~150 million kilometers) at 1950 UT on 11 Nov. 2020.
2020 VX5
© Remanzacco Blogspot

Laptop

With macOS Big Sur, your computer is already hacked

apple logo spying
© Notebookcheck.net
There have been several updates appended to this page as of 2020-11-16, please see below.

It's here. It happened. Did you notice?

I'm speaking, of course, of the world that Richard Stallman predicted in 1997. The one Cory Doctorow also warned us about.

On modern versions of macOS, you simply can't power on your computer, launch a text editor or eBook reader, and write or read, without a log of your activity being transmitted and stored.

It turns out that in the current version of the macOS, the OS sends to Apple a hash (unique identifier) of each and every program you run, when you run it. Lots of people didn't realize this, because it's silent and invisible and it fails instantly and gracefully when you're offline, but today the server got really slow and it didn't hit the fail-fast code path, and everyone's apps failed to open if they were connected to the internet.

Because it does this using the internet, the server sees your IP, of course, and knows what time the request came in. An IP address allows for coarse, city-level and ISP-level geolocation, and allows for a table that has the following headings:
Date, Time, Computer, ISP, City, State, Application Hash

Comment: So with this, not only can Apple, as well as government agencies, get information about you, but so can nefarious middle men, since your private data isn't even encrypted during transmission.

Who trusts Apple, anyway?


Robot

Robot patrols shop checking lockdown restrictions are being adhered to in Japan

robot
© NHK
The 'Robovie' robot is making customers in a shop in Japan keep to coronavirus measures.
A shop in Japan has enlisted a robot to ensure customers are wearing masks, as the country prepares for a possible third wave of coronavirus infections.

Robovie, developed by the Advanced Telecommunications Research Institute International in Kyoto, is able to pick out customers who aren't wearing masks and politely ask them to cover up. It can also intervene when they fail to socially distance while queuing up to pay.

The trial, which began last week at the club shop of Cerezo Osaka, a professional football team, will run until at least the end of the month.

Robovie's developers, who are behind a host of robotic innovations, hope the experiment will reduce close contact between shoppers and staff, adding that they believe most people will feel less embarrassed by being asked to cover up by a robot than by a fellow human being.

Comment: See also: